PMC

Specificity of S. agalactiae-MCDA-LFB assays. A total of 44 strains were used to evaluate the specificity of MCDA-LFB assays. 1, Streptococcus agalactiae strain THH-Sa001; 2–8, Streptococcus agalactiae; 9–10, Streptococcus pyogenes; 11–12, Streptococcus pneumoniae; 13–14, Streptococcus mitis; 15–16, Streptococcus salivarius; 17–18, Streptococcus sanguinis; 19, Streptococcus dysgalactiae; 20, Streptococcus gordonii; 21, Streptococcus sinesis; 22, Streptococcus contellatus; 23–24, Streptococcus anginosus; 25, Enterococcus faecalis; 26, Enterococcus faecium; 27, Enterococcus raffinosus; 28, Staphylococcus haemolyticus; 29, Staphylococcus hominis; 30, Staphylococcus aureus; 31, Staphylococcus epidemidis; 32, Staphylococcus saprophyticus; 33, Staphylococcus capitis; 34, Staphylococcus lugdunensis; 35, Micrococcus yunnanensis; 36, klebsiella pneumoniae; 37, Citrobacter freumdii; 38, Bacillus mirabilis; 39, Enterobacter gergoviae; 40, Enterobacter cloacae; 41, Escherichia coli; 42, Lactobacillus jensenii; 43, Candida albicans; 44, Candida tropicalis.

Specificity analysis. A total of 28 strains were used to evaluate the specificity of MCDA-LFB assays. Light green was visuable for all Streptococcus agalactiae, and colorless was observed for non-Streptococcus agalactiae. 1, Positive control (Streptococcus agalactiae strain ATCC12386), 2–7, Streptococcus agalactiae. 8, Streptococcus pyogenes; 9, Streptococcus pneumoniae; 10, Streptococcus mitis; 11, Streptococcus salivarius; 12, Streptococcus sanguinis; 13, Streptococcus dysgalactiae; 14, Streptococcus gordonii; 15, Streptococcus sinensis; 16, Streptococcus constellatus; 17, Streptococcus anginosus; 18, Enterococcus faecium; 19, Enterococcus raffinosus; 20, Staphylococcus epidermidis; 21, Staphylococcus saprophyticus; 22, Lactobacillus jensenii; 23, Micrococcus yunnanensis; 24, Pseudomonas aeruginosa; 25, klebsiella pneumoniae; 26, Escherichia coli; 27, Candida albicans; 28, Candida tropicalis

Morphology of C. albicans and C. tropicalis in the control and the test groups under OM observation. (Aa) C. albicans, 12 h; (Ab) intracellular protein-treated C. albicans, 12 h. (Ba) C. tropicalis, 12 h; (Bb) intracellular protein-treated C. tropicalis, 12 h. (Ca) C. albicans and C. tropicalis co-culture, 12 h; (Cb) intracellular protein-treated co-culture, 12 h. Magnification, ×1,000. C. albicans, Candida albicans; C. tropicalis, Candida tropicalis; OM, optical microscopy.

Morphology of C. albicans and C. tropicalis in the control and the test groups under SEM observation. (Aa) C. albicans, 12 h; (Ab) intracellular protein-C. albicans, 12 h (magnification, ×4,000). (Ba) C. tropicalis, 12 h; (Bb) intracellular protein-C. tropicalis, 12 h (magnification, ×2,000). (Ca) C. albicans and C. tropicalis co-cultures, 12 h; (Cb) intracellular protein-co-cultures, 12 h (magnification, ×2,000). C. albicans, Candida albicans; C. tropicalis, Candida tropicalis; SEM, scanning electron microscopy.